最近在瞎翻译一些本科毕业做的内容，主要探究一个叫ANP3A的基因对鸡马立克氏病肿瘤细胞生长的影响。如果大家能一起把对肿瘤细胞有影响的那个基因揪出来，那么小鸡们就能更快乐成长，少生病了!
本文专业词汇比较多，而且语法不太行....所以战友不必过于认真阅读呀，有兴趣的话可以读一读我前几篇文章，是我现在的研究领域。链接如下:
Introduction:
Marek’s disease (MD) is a T cell lymphoma disease of domestic chickens induced by the Marek’s disease virus (MDV), a highly infectious and naturally oncogenic alphaherpesvirus. Enhancing genetic resistance to MD in poultry is an attractive method to augment MD vaccines, which protect against MD but do not prevent MDV replication and horizontal spread. MicroRNAs (miRNAs) are short noncoding RNAs that act as post-transcriptional regulators. They can effectively silence target genes by binding to the 3′-untranslated region (3′-UTR) of target mRNAs, causing mRNA degradation or inhibiting translation . Indeed, these miRNAs sometimes exert a role as oncogenes or tumor suANPressors by affecting the response to various therapeutic interventions. MiR-181a (59-AACAUUCAACGCUGUCGGUGAGU-39) is a key modulator of cellular differentiation, including hematopoietic lineage differentiation, myoblast differentiation, and Tcell sensitivity and selection. Acidic leucine-rich nuclear phosphoprotein 32A (ANP32A) contains an Nterminal leucinerich repeat (LRR) domain. ANP32A acts as a tumour suANPressor and its expression is altered in many tumours. In addition, ANP32A is involved in other activities such as modulating histone acetylation and transcription as it forms part of the INHAT (inhibitor of histone acetyltransferases) complex[4]. It also participates in other biochemical processes such as regulation of mRNA trafficking and stability by its association with HuR (Huantigen R), which increases the stability of mRNA binding to the 30-untranslated region (UTR) region.
In the preliminary study, we found a number of miRNAs related to Marek's disease (MD tumor formation) by Solexa deep sequencing and verified that ANP32A was a target gene of gga-mi-181a. Based on this result, the aim of this article is to verify the effect of ANP32A on Marek's disease tumor transformation by designing three siRNAs transfected in MSBcells and to interfere with the expression of ANP32A gene.
ANP32A plays an important regulatory role in a variety of life activities, such as cell proliferation, apoptosis, and transcriptional regulation. In recent years, more and more studies have shown that ANP32A has effect on the occurrence and development of tumor diseases and participates in the regulation of various malignant tumor diseases.
After over-expression of pp32 (ANP32A) in human hepatocellular carcinoma cell line HepG2, compared with the negative control group, the cloning ability of HepG2 cells was not significantly different. Other studies have shown that after introducing the pp32 gene into human liver cancer cells HepG2, the expression of the pp32 gene changes the morphology of liver cancer cells, but has no significant effect on the proliferation of liver cancer cells . In this study, siRNA was used to interfere with the expression of ANP32A gene. Compared with the control group, there was no significant difference in the proliferation of MSB-1 cells. Therefore, it can be considered that the expression of ANP32A has no significant effect on the proliferation of MSB-1 cells. In many tumor diseases, exploring the effect of ANP32A gene on tumor cell proliferation is of great significance for the study of tumor development, diagnosis and treatment.
The cell cycle divided into five periods, namely: G0 phase, G1 phase, S phase, G2 phase and M phase is a complete life process of a cell.The effect of ANP32A gene on different periods of cell cycle can be tested to verify its role in cell proliferation and differentiation. Studies have shown that in the pancreatic cancer MaiPaCa2 cell line, overexpression of ANP32A affects the cell cycle by blocking the occurrence of the G1 phase, thereby affecting the cell growth process. In this study, compared with the control group, after transfection of siRNA to reduce the expression of ANP32A, there were no significant differences in the proportion of cells in the G0, G1, S, G2, and M phases of the cell cycle, indicating that interference with ANP32A gene on MS tumor cells There was no significant blocking or promoting effect in 1 cycle.
Apoptosis is the last process in the life course of a cell, that is, the spontaneous death of a cell, which is regulated by a combination of various factors. In a variety of tumor diseases, the apoptosis of tumor cells plays an important role in inhibiting the development of tumor diseases, which can provide new ideas for the diagnosis and treatment of diseases.
At present, Marek's disease is still a potential hazard in the poultry industry, which will lead to a decline in poultry production capacity, an increase in mortality, and immunosuppression, resulting in serious economic losses. Along with the continuous development of science and the improvement of breeding technology, a large number of vaccines against Marek's disease have been gradually developed, and Marek's disease is controlled during the production process. However, currently widely used vaccines still cannot fully prevent Marek's virus infection, and the use of vaccines has the potential to promote the evolution of virus virulence. Therefore, revealing the host's own resistance and susceptibility factors provides new ideas for the prevention and treatment of Marek's disease in chickens.
