因为权限问题安装失败
大意是：错误 1303。安装程序没有足够的权限来访问C:\programData\Microsoft\Windows\Start Menu\Programs\Autodesk这个目录。安装无法继续。以管理员身份登录，或与您的系统管理员联系。
不会吧！我Windows 8还有这嗜好？！立马启动内置的 administrator 后，重启电脑，使用 administrator 大法安装。居然还是这脸色！神马玩意？！
仔细研究，多次重启，终于搞定！办法如下：
1、打开C:\programData\Microsoft\Windows\Start Menu\文件夹，找到Programs；
2、右击Programs-->属性，切换到“安全”标签；
3、点击“编辑”按钮-->“添加”-->“高级”-->“立即查找”，在搜索结果栏中找到SYSTEM-->“确定”-->“确定”；
4、然后在次安装就可以了
识别不了USB
在运行中输入services.msc
找到VMwaer USB 开头的文件，全部点启动
安装VMware tools
打开虚拟机后，点击 “设置——安装VMware tools”，然后点 “是”，然后虚拟机里面就有了VMware tools 的压缩包，移到home目录下
tar -zxvf VMwareTools-9.2.2-893683.tar.gz
cd vmware-tools-distrib
sudo perl vmware-install.pl
#在安装之前，可能需要安装一些软件
#sudo apt install cmake
#sudo apt install net-tools
就可以了
安装aspera
wget -c https://d3gcli72yxqn2z.cloudfront.net/connect/bin/ibm-aspera-connect-3.8.1.161274-linux-g2.12-64.tar.gz
tar -zxvf ibm-aspera-connect-3.8.1.161274-linux-g2.12-64.tar.gz
#https://download.asperasoft.com/download/sw/connect/3.9.9/ibm-aspera-connect-3.9.9.177872-linux-g2.12-64.tar.gz
#2020.5.21
mkdir biosoft
mv ibm-aspera-connect-3.8.0.158533-linux-g2.12-64.sh biosoft/
cd biosoft/
bash ibm-aspera-connect-3.8.0.158533-linux-g2.12-64.sh
cd
echo export PATH="/home/u1013/.aspera/connect/bin:$PATH" >>.bashrc
使用aspera下载文件
for i in `seq 00 03`
do
ascp -T -l 200M -i ~/.aspera/connect/etc/asperaweb_id_dsa.openssh --host=ftp-private.ncbi.nlm.nih.gov --user=anonftp --mode=recv /sra/sra-instant/reads/ByRun/sra/SRR/SRR402/SRR40223$i/SRR40223$i.sra ./
done
在使用的过程中可能遇到网络问题
参考：http://download.asperasoft.com/download/docs/proxy/1.2.2/admin_linux/webhelp/dita/troubleshooting.html
Session Stop (Error: Client unable to connect to server (check UDP port and firewall))
If you get this error, check the following:
- Ensure that IP forwarding is enabled. IP forwarding must be enabled and is enabled automatically when FASP Proxy is installed. To confirm, run the following command:
cat /proc/sys/net/ipv4/ip_forward
If the command returns 1, IP forwarding is enabled. If it returns 0, it is not. IP forwarding can be enabled manually by setting the **net.ipv4.ip_forward** line in **/etc/sysctl.conf** as follows:
# Controls IP packet forwarding
net.ipv4.ip_forward=1
To activate changes to /etc/sysctl.conf, run the following:
/sbin/sysctl -p /etc/sysctl.conf
- If the error still occurs when IP forwarding is on, open your aspera.conf file and turn off source-port filtering as follows. By default, source-port filtering is enabled.
...
<rproxy>
<enabled>false</enabled>
...
<rules>
<rule>
...
<src_port_filtering>false</src_port_filtering>
</rule>
</rules>
</rproxy>
...
If the same timeout errors still occur when source-port filtering is disabled, this generally indicates that traffic is being blocked at a firewall.
For more information about source-port filtering, see Source-Port Filtering.
安装conda
wget -c https://repo.continuum.io/miniconda/Miniconda3-latest-Linux-x86_64.sh
sh Miniconda3-latest-Linux-x86_64.sh #全部选择yes，就将conda添加到环境变量了，有时需要使用bash代替sh
source ~/.bashrc
添加channels
conda config --add channels r
conda config --add channels defaults
conda config --add channels conda-forge
conda config --add channels bioconda
添加国内channels
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/conda-forge/
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/free/
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/bioconda/
#上面的是清华大学的源，但是似乎现在不是太好用了，可以试试中科大的源
conda config --add channels https://mirrors.ustc.edu.cn/anaconda/pkgs/free/
上下左右键变成ABCD
cp /etc/vim/vimrc ~/.vimrc
将软件添加到环境变量
export PATH=$PATH:/opt/au1200_rm/build_tools/bin #就是将软件的bin目录添加到环境变量
或是直接在bashrc里面进行修改
vi .bashrc
export PATH="/opt/au1200_rm/build_tools/bin:$PATH"
然后source一下
source .bashrc
