conda 下安装gfold
conda activate python27
conda install -c bioconda gfold
###安装后 ，gfold -h出现报错：Error while loading shared libraries: libgsl.so.0: cannot object file: No such fileopen shared object file: No such file or directory
解决：
- gfold 依赖于gsl库，先确认好已安装好gsl相关软件，which gsl查找一下；
- 查找到已安装过gsl, ln -s软连接到conda 指定环境下的share文件夹，并重命名gsl为 libgsl.so.0；
- 确定没有安装，安装一下，再重复2的动作
下载gsl软件包
wget -c http://mirrors.ustc.edu.cn/gnu/gsl/gsl-2.4.tar.gz
##解压
tar -zxvf gsl-2.4.tar.gz
进入软件目录开始安装
cd /home/u20111230014/workspace/software/gsl-2.4
###先configure
./configure --prefix=/home/u20111230014/workspace/software/gsl-2.4
##编译：
make
###安装库之前测试一下是否make成功：
make check
PS:若make 或 make check报错:fatal error: gsl/gsl_minmax.h: No such file or directory
则修改文件夹gsl-2.4为gsl, 再重新make，再make check
###安装库：make install
###最后所有都没报错后，重新configure一次
./configure --prefix=/home/u20111230014/workspace/software/gsl-2.4
##配置环境变量，添加以下入：vim ~/.bashrc
export C_INCLUDE_PATH=$C_INCLUDE_PATH:/home/u20111230014/workspace/software/gsl-2.4/include
export CPLUS_INCLUDE_PATH=$CPLUS_INCLUDE_PATH:/home/u20111230014/workspace/software/gsl-2.4
export LD_LIBRARY_PATH=$LD_LIBRARY_PATH::/home/u20111230014/workspace/software/gsl-2.4/lib
export LIBRARY_PATH=$LIBRARY_PATH::/home/u20111230014/workspace/software/gsl-2.4/lib
###测试安装是否成功
##输入两句，分别生成test.o 和a.out，出现 a.out执行文件则成功
gcc -Wall -I/home/u20111230014/workspace/software/gsl-2.4/include -c test.c
chmod 755 test.c
gcc -L/home/u20111230014/workspace/software/gsl-2.4/lib test.o -lgsl -lgslcblas -lm
#
##软连接到conda指定环境下的lib和share文件夹
/home/u20111230014/miniconda3/envs/python27/lib
/home/u20111230014/miniconda3/envs/python27/share/:
ln -s /home/u20111230014/workspace/software/gsl-2.4/lib/libgsl.so.23.0.0
ln -s /home/u20111230014/workspace/software/gsl-2.4/lib/libgsl.so.23.0.0 libgsl.so.0
必须两个文件夹均有软连接，不然重启conda 后又会一样报错
唤醒软件
gfold -h
===============================================================================
gfold : Generalized fold change for ranking differentially expressed
genes from RNA-seq data.
Author : Jianxing Feng (jianxing.tongji@gmail.com)
Date : Sun May 24 07:42:36 CST 2015
Version : V1.1.4
===============================================================================
USAGE: Please go to the source code directory and use command 'man doc/gfold.man'
or open doc/gfold.html to find documentation.
Quick Examples:
gfold count -ann hg19Ref.gtf -tag sample1.sam -o sample1.read_cnt
gfold count -ann hg19Ref.gtf -tag sample2.sam -o sample2.read_cnt
gfold diff -s1 sample1 -s2 sample2 -suf .read_cnt -o sample1VSsample2.diff
参考：
https://blog.csdn.net/u012248802/article/details/80655902
