1. Cellranger的下载
此处以10X官网上最新的Cellranger版本为例下载，网址如下：
wget -O cellranger-6.1.2.tar.gz "https://cf.10xgenomics.com/releases/cell-exp/cellranger-6.1.2.tar.gz?Expires=1635685923&Policy=eyJTdGF0ZW1lbnQiOlt7IlJlc291cmNlIjoiaHR0cHM6Ly9jZi4xMHhnZW5vbWljcy5jb20vcmVsZWFzZXMvY2VsbC1leHAvY2VsbHJhbmdlci02LjEuMi50YXIuZ3oiLCJDb25kaXRpb24iOnsiRGF0ZUxlc3NUaGFuIjp7IkFXUzpFcG9jaFRpbWUiOjE2MzU2ODU5MjN9fX1dfQ__&Signature=IdVssu4uhoH2HQcJ6Fc66z~Wcf8x09lfKU0g2ARgKBjB11bc3IroxYh3ZeExzOJzZkTNjuIo94pRmjMZ9GjjIzOwteLBmKU83nA2JUbUPW28CAfvWFiuTDme94Ty68XUuYEXNPYJA88YP3OyMGgh2T4KKAdYRen2GDjmu~MvUZdNtOniTsWNbT0IQBeoGI8DE1Rcl1QLiK90uiCcw-aXOsTc~w4OQZiEUj0vd5N~KgpUWHdd4Nzy9Y0~SP~DBFkSYYcKEZ6qOUnq~BUDOq5QMcsPfz244WNaFfnUkLnF2tnzXQsjYvDm7obs2iWUGRXgnODcJ7B9UXRrLG5wDfdI7A__&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA"
下载至某一文件夹，本例中以下载至software文件夹为例。
cd ~/software
之后，进行解包、解压缩。
tar -zxvf cellranger-6.1.2.tar.gz
2. 添加Cell Ranger到$PATH
export PATH=~/download/cellranger-6.1.2:$PATH
检验是否添加到$PATH:
which cellranger
输出如下：
/home/user_name/software/cellranger-6.1.2/cellranger # user_name是所使用服务器用户的名字
也可以添加到.bashrc，这样下次登陆就不用再次export了，切换到家目录下,
vi .bashrc
将下方这句添加到.bashrc文件中，
export PATH=~/download/cellranger-6.1.2:$PATH
更新一下.bashrc文件。
source .bashrc
3. 执行 sitecheck
cellranger sitecheck > sitecheck.txt
发送sitecheck.txt到10X的支持团队
cellranger upload your_email@email.com sitecheck.txt
输出如下，
Getting upload information...
Uploading file...
Size: 17,676 bytes
100% [=================================>] 17,676 0.28Mb/s eta 0m 0s
Upload complete!
4. Cellranger试运行
cellranger testrun --id=tiny
试运行结果输出如下，
Pipestance completed successfully!
