这次想好了好好学习，下来血本，按照推荐配了一台个人的服务器。这周刚刚点亮。工作站主机E5-2683 V3正式版CPU28核56线程双路E5服务器。
主板：超微X10DAI
CPU： E5-2683 V3 x2
内存：三星 REG DDR4 16G
硬盘：2T 机械硬盘
亮卡机：GT 610 淘宝二手65元包邮
组装好后，快递回来的。这部分的费用是10200+65￥的费用。。。
回来买了一台27寸的三星显示器 1599￥，买了VGI的连接线35。。。
借着下班回来的的短暂时间，试了机器感觉还是很不错。回来点亮了机器，诧异的是插上网线，并未填写过密码，电脑直接就可以联网了。一起下来12000￥。刚刚开始还是不习惯linux的系统。不停地在笔记本上和台式的linux系统上不停地切换。
按照Bio-star上的说明。运行了doctor.py.
最开始的15个13个显示error。反复倒腾过后开始慢慢了解怎么安装转件。现在还在9个error。其实家里的网也不慢，发现linux的下载速度都是超级慢，再装hisat2时，只有32M，却下载了整整80min。简直罪过了。以前只知道找分析的人要什么样的图，现在发现大家都不容易。干分析和做实验还是很像的，都有一个protocol，一步一步慢慢走才行。目前我按还是照生信媛的biostar课程慢慢开始吧。
admin1@hyl:~$ mkdir -p ~/bin
admin1@hyl:~$ curl http://data.biostarhandbook.com/install/doctor.py>~/bin/doctor.py
% Total % Received % Xferd Average Speed Time Time Time Current
Dload Upload Total Spent Left Speed
100 3570 100 3570 0 0 976 0 0:00:03 0:00:03 --:--:-- 976
admin1@hyl:~$ chmod +x ~/bin/doctor.py
admin1@hyl:~$ ~/bin/doctor.py
# Doctor! Doctor! Give me the news.
# The ~/bin folder is not in your PATH!
# Checking 15 symptoms...
# Missing program: bwa
# Missing program: datamash
# Missing program: fastqc
# Missing program: hisat2
# Missing program: seqret
# Missing program: subread-align
# Missing program: featureCounts
# Missing program: R
# Missing program: efetch
# Missing program: esearch
# Missing program: samtools
# Missing program: fastq-dump
# Optional program not found: wonderdump
# Optional program not found: global-align.sh
# Optional program not found: local-align.sh
# Your system shows 13 errors.
# See also: doctor.py --fixme
admin1@hyl:~$ PATH=$PATH:~/bin
admin1@hyl:~$ doctor.py --fixme
cd ~/biosoft
mkdir HISAT&&cd HISAT
wget ftp://ftp.ccb.jhu.edu/pub/infphilo/hisat2/downloads/hisat2-2.0.4-Linux_x86_64.zip
unzip hisat2-2.0.4-Linux_x86_64.zip
ln -s hisat2-2.0.4 current
cd ~/biosoft
mkdir HTSeq&&cd HTSeq
wget https://pypi.python.org/packages/72/0f/566afae6c149762af301a19686cd5fd1876deb2b48d09546dbd5caebbb78/HTSeq-0.6.1.tar.gz
tar zxvf HTSeq-0.6.1.tar.gz
cd HTSeq-0.6.1
python setup.py install --user
~/.local/bin/htseq-count --help
admin1@hyl:~$ doctor.py
# Doctor! Doctor! Give me the news.
# Checking 15 symptoms...
# Missing program: bwa
# Missing program: datamash
# Missing program: fastqc
# Missing program: hisat2
# Missing program: seqret
# Missing program: subread-align
# Missing program: featureCounts
# Missing program: samtools
# Missing program: fastq-dump
# Optional program not found: wonderdump
# Optional program not found: global-align.sh
# Optional program not found: local-align.sh
# Your system shows 9 errors.
# See also: doctor.py --fixme
admin1@hyl:~$ doctor.py --fixme
