文章参考：
实战
1.加载数据：
options(BioC_mirror="https://mirrors.tuna.tsinghua.edu.cn/bioconductor")
options("repos"=c(CRAN="https://mirrors.tuna.tsinghua.edu.cn/CRAN/"))
rm(list = ls()) ## 魔幻操作，一键清空~
options(stringsAsFactors = F)
load('GSE9257_lasso.Rdata')
###提取IL13RA2, CDH3 and COMP
load("myoverlap.Rdata")
rt=as.data.frame(t(exprSet[myoverlap,]))
rt$group=ifelse(meta_GSE9257_lasso$event==1,"IPF","Control")
x=colnames(rt)[4]
2.数据准备：
###加载需要的包
library(reshape2)
library(tidyr)
library(ggplot2)
library(ggpubr)
###数据转换：
#把数据转换成gglpot2输入文件
data=melt(rt,id.vars=c("group")) ##宽数据变长数据
colnames(data)=c("group","Gene","Expression")
head(data)
3.做图
###绘制图形
#绘制boxplot
p=ggboxplot(data, x="Gene", y="Expression", color = "group",
ylab="Gene expression",
xlab="",
legend.title=x,
palette = c("blue","red"),
width=0.6, add = "none")
p=p+rotate_x_text(60)
p1=p+stat_compare_means(aes(group=group),
method="t.test",
symnum.args=list(cutpoints = c(0, 0.001, 0.01, 0.05, 1),
symbols = c("***", "**", "*", " ")),
label = "p.signif")
#输出图片
#pdf(file=outFile, width=6, height=5)
print(p1)
小提琴图：
# plot
ggplot(data,aes(x = Gene, y = Expression,fill = group)) +
# 小提琴图层
geom_violin(position = position_dodge(0.9),alpha = 0.5,
width = 1.2,trim = T,
color = NA) +
# 箱线图图层
geom_boxplot(width = .2,show.legend = F,
position = position_dodge(0.9),
color = 'grey20',alpha = 0.5,
outlier.color = 'grey50') +
# 主题调整
theme_bw(base_size = 14) +
theme(axis.text.x = element_text(angle = 0,color = 'black'),#,hjust = 1
legend.position = 'top',
aspect.ratio = 0.8) + ##图形长宽比例
# 颜色设置
scale_fill_manual(values = c('Control'='#398AB9','IPF'='red'),
name = '') +
# 添加显著性标记
stat_compare_means(aes(group=group),
method="t.test",
symnum.args=list(cutpoints = c(0, 0.001, 0.01, 0.05, 1),
symbols = c("***", "**", "*", "NS")),label = "p.signif",
label.y = 8,size = 5) +
#ylim(0.5,3.5)
###添加标题
labs(x="GSE9257")+
theme(axis.title.x = element_text(color="black", size=12, face="bold")
)
###添加标题：
p +labs(title="Plot of length \n by dose",
x ="Dose (mg)", y = "Teeth length")+
theme(
plot.title = element_text(color="red", size=12, face="bold.italic"),
axis.title.x = element_text(color="blue", size=12, face="bold"),
axis.title.y = element_text(color="#993333", size=12, face="bold")
)
