1.MultiQC安装
pip install multiqc
报错:
-
distributed 1.21.8 requires msgpack, which is not installed.
缺少msgpack,输入pip install msgpack
即可。 -
Cannot uninstall 'PyYAML'. It is a distutils installed project and thus we cannot accurately determine which files belong to it which would lead to only a partial uninstall.
进入anaconda目录(为了防止删掉其他无辜文件),然后执行
find '.' -name "*PyYAML*.egg-info" -exec rm -rf {} \;
(引自https://blog.csdn.net/j___t/article/details/96052936)
2.从网上下载fastq文件
prefetch SRR7511258
prefetch SRR6232298
3.将下载的文件转换成fastq格式
fastq-dump --gzip --split-files SRR7511258.sra
fastq-dump --gzip --split-files SRR6232298.sra
4.用fastqc评价
fastqc SRR6232298_1.fastq.gz SRR6232298_2.fastq.gz SRR9111834_1.fastq.gz SRR9111834_2.fastq.gz
5.multiqc报告解读
输入代码multiqc .
打开html,结果如下: