阅读文献Chromatin accessibility changes between Arabidopsis stem cells and mesophyll cells illuminate cell type-specific transcription factor networksATAC数据处理完之后下游所用工具
1.deeptools可视化画热图,所用子命令bamcoverage和comuterMatix
2.对THSs(超敏感位点)进行peakcalling:Homer,Findpeaks
3.THSs基因组的分布:PAVIS(在线网络工具)
4.对于富含特定细胞类型的TS,HTseq的htseq-count或Deseq2(p值<=0.05)
5.对转录因子的基序分析:RAST,DREME,MEME
6.将THSs分配给附近的基因:PeakAnnotator:TSS
7.蛋白相互作用分析:STRING
8.定义转录因子的预测结合位点:FIMO
9.基因本体分析:AgriGO,GO Analysis Toolkit